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The following techniques
are used for our single molecule experiments:
- Scanning Confocal Fluorescence
Microscopy (SCFM)
- Total Internal Reflection Fluorescence
Microscopy (TIRFM)
- Simultaneous measurement of Atomic
Force Microscopy (AFM) images and single molecule fluorescence images
- Fluorescence Lifetime Imaging (FLIM)
and Time Gated Imaging (TGI)
- Transmission Electron Microscopy (TEM)
Data are
analyzed using the following techniques:
- Förster Resonance Energy Transfer (FRET)
Efficiency
- Fluorescence Correlation Spectroscopy (FCS)
- Image Correlation Spectroscopy (ICS)
- Statistical analysis of time durations
(Histogram binning)
- Colocalization and Cross-Correlation
analysis
- Single Particle Tracking (SPT)
Our 4-channel
Scanning Confocal Fluorescence Microscope with pulsed picosecond lasers for
fluorescence lifetime imaging.
Picoquant
Microtime 200
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